The Department of Chemical and Systems Biology Presents

Professor Alanna Schepartz
T.Z. and Irmgard Chu Distinguished Chair in Chemistry
Professor of Molecular and Cell Biology
Faculty Affiliate, California Institute for Quantitative Biosciences (QB3)
University of California, Berkeley

Talk Title: “Protein Delivery to Cells and Animals”

Abstract: I will describe the discovery of a miniature protein that guides proteins and enzymes into the cell interior by promoting endosomal escape, a single-molecule tool that quantifies this trafficking event in live cells with accuracy and precision, experiments to probe how endosomal escape occurs, and how this knowledge can be applied for (cytosolic) enzyme replacement therapy.

Reading Material:
1. Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic, Delivery of Protein Cargo by Cell-Permeant Miniature Proteins
Rebecca F. Wissner, Angela Steinauer, Susan L. Knox, Alexander D. Thompson, and Alanna Schepartz
ACS Central Science 2018 4 (10), 1379-1393, https://pubs.acs.org/doi/pdf/10.1021/acscentsci.8b00446

2. HOPS-dependent endosomal fusion required for efficient cytosolic delivery of therapeutic peptides and small proteins
Angela Steinauer, Jonathan R. LaRochelle, Susan L. Knox, Rebecca F. Wissner, Samuel Berry, and Alanna Schepartz
PNAS January 8, 2019 116 (2) 512-521; first published January 4, 2019; https://doi.org/10.1073/pnas.1812044116