CSB Cutting Lecture Series: “Splicing regulation at the U2 snRNP-branchpoint complex” Douglas Black, PhD, February 14, 2025, Munzer Auditorium!
Cutting Lecture Series
Douglas Black, Ph.D.
Professor, Department of Microbiology,
Immunology, and Molecular Genetics, UCLA
Friday, February 14, 2025
12:30 PM – 1:30 PM (Pacific Time)
Munzer Auditorium
Talk Title: “Splicing regulation at the U2 snRNP-branchpoint complex”
Talk Abstract: I will present new data examining how the interaction between the U2 snRNP and the intron branchpoint within the spliceosome contributes to regulation of spliced isoform choice and to mis-splicing in diseases such as Myelodysplastic Syndromes. A new procedure for isolating pre-mRNA-bound U2 and a new method of genomic analysis that generates maps of U2 binding across the transcriptome will be described. Proteins associated with U2 in these isolates include new regulatory factors. The structure of these complexes suggests a new model for regulatory protein action on the spliceosome by selective recruitment of an RNA helicase to disrupt the interaction of the intron branchpoint with the U2 snRNA.